![]() ![]() We used this setup to quantify membrane fusion by several well-studied mechanisms, including fusion triggered by Ca 2+, polyethylene glycol, and biospecific tethering. ![]() We investigated the effects of fusogenic agents by simply flowing these molecules into the chambers and analyzing the resulting shape changes of more than 100 liposomes in parallel. Using electroformation from spin-coated films of lipids on transparent indium tin oxide electrodes, we formed two-dimensional networks of closely packed, surface-attached giant liposomes. ![]() We present a method that makes it possible to trigger, observe, and quantify membrane aggregation and fusion of giant liposomes in microfluidic chambers. ![]()
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